Targeted silencing of CDX2 gene with siRNA enhanced vincristine-induced inhibition of proliferation and induction of apoptosis of leukemia K562 cells

Purpose: To determine the effect of targeted silencing CDX2 with siRNA on vincristine-induced inhibition proliferation and apoptosis induction in leukemia K562 cells.
 Methods: cells were divided into untreated group, vincristine blank CDX2-siRNA group. The expression gene after transfection was determined by reverse-transcription-polymerase chain reaction (RT-PCR) immunoblotting, while MTT flow cytometric procedures used to evaluate apoptotic changes Results: Protein did not change significantly non-treated cells, group protein decreased (p < 0.05) assay results showed that absorbance lower than those other three groups at 12, 24 48 h silencing. Flow cytometry markedly higher percentage There no difference level between but these 0.05).
 Conclusion: Silencing via targeting enhances suppression growth thereby enhancing anti-tumor vincristine.